Pepper variety nun 89007 pps

ABSTRACT

The disclosure provides a new and distinct hybrid variety of pepper, NUN 89007 PPS as well as seeds and plants and fruits thereof.

CROSS REFERENCE TO RELATED APPLICATION

The application claims priority to U.S. Provisional Application No.62/667,156, filed May 4, 2018, which is hereby incorporated by referencein its entirety.

FIELD OF THE DISCLOSURE

The disclosure relates to the field of plant breeding and, morespecifically, to the pepper variety NUN 89007 PPS. The disclosurefurther relates to vegetative reproductions of NUN 89007 PPS, methodsfor tissue culture of NUN 89007 PPS, and methods for regenerating aplant from such a tissue culture and also to phenotypic variants of NUN89007 PPS.

BACKGROUND

The goal of plant breeding is to combine various desirable traits in asingle variety/hybrid. Such desirable traits may include greater yield,resistance to diseases, insects or other pests, tolerance to heat anddrought, better agronomic quality, higher nutritional value, enhancedgrowth rate and improved fruit properties.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same genotype. A plant cross-pollinates if pollen comes to it from aflower of a different genotype. Plants that have been self-pollinatedand selected for (uniform) type over many generations become homozygousat almost all gene loci and produce a uniform population of truebreeding progeny of homozygous plants. A cross between two suchhomozygous plants of different lines produces a uniform population ofhybrid plants that are heterozygous for many gene loci. The extent ofheterozygosity in the hybrid is a function of the genetic distancebetween the parents. Conversely, a cross of two plants each heterozygousat a number of loci produces a segregating population of hybrid plantsthat differ genetically and are not uniform. The resultingnon-uniformity makes performance unpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants to makehybrids, and the evaluation of the hybrids resulting from the crosses.Pedigree breeding and recurrent selection are examples of breedingmethods that have been used to develop inbred plants from breedingpopulations. Those breeding methods combine the genetic backgrounds fromtwo or more plants or various other broad-based sources into breedingpools from which new lines are developed by selfing and selection ofdesired phenotypes. The new plants are evaluated to determine which havecommercial potential.

One crop species that has been subject to such breeding programs and isof particular value is the pepper. Pepper (Capsicum spp.) is naturally adiploid and the basic chromosome number of the genus is x=12, most are2n=2x=24, including the cultivated ones. A few wild species have 2n=26.Ploidy changes (both tetraploidy and haploidy) are relatively easy toinduce in Capsicum species. Doubled haploids have proved particularlyvaluable in the analysis of the genetically complex basis of someresistances to pests and diseases.

The genus Capsicum originated in American tropics. The fruit of mostspecies of Capsicum produce a strong burning sensation (pungency orspiciness) in the mouth of the unaccustomed eater due to the presence ofcapsaicin (methyl vanillyl nonenamide), a lipophilic chemical, making itas an important spice commodity. Capsaicin can be present in largequantities in the placental tissue (which holds the seeds), the internalmembranes, and to a lesser extent, the other fleshy parts of the fruitsof plants in this genus. The seeds themselves do not produce anycapsaicin. The amount of capsaicin in the fruit is highly variable anddependent on genetics and environment, giving almost all types ofCapsicum varied amounts of perceived heat.

The most recognizable Capsicum without capsaicin is the bell pepper, acultivar of Capsicum annuum, which has a zero rating on the Scovillescale. The lack of capsaicin in bell peppers is due to a recessive genethat eliminates capsaicin and, consequently, the “hot” taste usuallyassociated with the rest of the Capsicum family.

Pepper can be classified according to its target market: fresh marketand processing peppers. Peppers for the fresh market require that thefruits are firm, shiny and have fresh green calyx and stem. They aretypically consumed fresh as a snack or used in salad or sandwiches or asa cooked vegetable. On the other hand, processing peppers are used forfreezing or dehydrating and can be dried, ground as spices andprocessed, e.g., pickled, canned, brined or in salsas.

In the USA, majority of the peppers produced is the bell pepper (i.e.,sweet pepper), which are mainly marketed fresh. California and Floridaare the lead producers of bell peppers. Bell peppers are availableyear-round with supply at the greatest volume from May to July and Marchto April in California and Florida, respectively. Bulk of the bellpeppers grown and harvested are green, but premium is given to coloredbell peppers (i.e., red, yellow).

Both hybrids and open-pollinated varieties are used for production inthe US, with a growing trend in the use of seeded hybrid varieties.Hybrid varieties offer the advantages of easy combination of dominantand recessive traits, such as disease resistance, from a set of inbredparents, as well as careful control of parentage.

Advances in biotechnology have also resulted in genetically engineeredpepper plants with improved traits. For example, fungal resistant pepperplants comprising a PepEST or PepDef gene where the expression of thenucleic acid sequence in the plant resulted in increased resistance tofungal infection, see e.g., U.S. Pub. No. 2006/0037100, which herebyincorporated by reference in its entirety.

While breeding efforts to date have provided a number of useful peppervarieties with beneficial traits, there remains a great need in the artfor new varieties with further improved traits. Such plants wouldbenefit farmers and consumers alike by improving crop yields and/orquality. Breeding objectives include resistance to pests and diseases,improvement of fruit quality, protection against biotic and abioticstresses, varying the color, texture and flavor of the fruit, optimizingflesh thickness, yield, suitability to various climatic circumstances,heat, solid content (% dry matter), or sugar content.

SUMMARY OF VARIOUS ASPECTS OF THE DISCLOSURE

The disclosure provides for a pepper variety NUN 89007 PPS, productsthereof, and methods of using the same. NUN 89007 PPS is an orange, MiniSweet Pointed sweet pepper for the snacking segment. It is suitable forearly harvest with concentrated fruit set.

In one aspect, the disclosure provides, a seed of pepper variety NUN89007 PPS, wherein a representative sample of said seed has beendeposited under Accession Number NCIMB 43268. The disclosure alsoprovides for a plurality of seeds of NUN 89007 PPS. The pepper seed ofNUN 89007 PPS may be provided as an essentially homogeneous populationof pepper seed. The population of seed of NUN 89007 PPS may beparticularly defined as being essentially free from other seed. The seedpopulation may be grown into plants to provide an essentiallyhomogeneous population of pepper plants as described herein.

The disclosure also provides a plant grown from a seed of pepper varietyNUN 89007 PPS and a plant part thereof. In another aspect, thedisclosure provides for a hybrid variety of pepper called NUN 89007 PPS.The disclosure also provides for a progeny of NUN 89007 PPS. In anotheraspect, the disclosure provides a plant or a progeny retaining all orall but one, two or three of the “distinguishing characteristics” or allor all but one, two or three of the “morphological and physiologicalcharacteristics” of NUN 89007 PPS and methods for producing that plantor progeny.

In one aspect, the disclosure provides a plant or a progeny having allthe physiological and morphological characteristics of variety NUN 89007PPS when grown under the same environmental conditions. In anotheraspect, the plant or progeny has all or all but one, two or three of thephysiological and morphological characteristics of NUN 89007 PPS whenmeasured under the same environmental conditions and e.g., evaluated atsignificance levels of 1%, 5% or 10% significance (which can also beexpressed as a p-value) for quantitative characteristics, wherein arepresentative sample of seed of variety NUN 89007 PPS has beendeposited under Accession Number NCIMB 43268. In another aspect, theplant or progeny has all or all but one, two or three of thephysiological and morphological characteristics as listed in Table 1and/or 2 and/or 3 for variety NUN 89007 PPS when measured under the sameenvironmental conditions and e.g., evaluated at significance levels of1%, 5% or 10% significance (which can also be expressed as a p-value)for quantitative characteristics.

In another aspect, a plant of NUN 89007 PPS or a progeny thereof has 7,8, or more or all of the following distinguishing characteristics asshown in Table 1 and/or 2: 1) ______; 2) ______; 3) ______; 4) ______;5) ______; 6) ______; 7) ______; 8) ______; 9 ______ : and 10) ______.

The disclosure provides for a plant part obtained from variety NUN 89007PPS, wherein said plant part is: a fruit, a harvested fruit, a part of afruit, a leaf, a part of a leaf, pollen, an ovule, a cell, a petiole, ashoot or a part thereof, a stem or a part thereof, a root or a partthereof, a root tip, a cutting, a seed, a part of a seed, seed coat oranother maternal tissue which is part of a seed grown on said variety,hypocotyl, cotyledon, a scion, a stock, a rootstock, a pistil, ananther, or a flower or a part thereof. Fruits are particularly importantplant parts. In a further aspect, the plant part obtained from varietyNUN 89007 PPS is a cell, optionally a cell in a cell or tissue culture.That cell may be grown into a plant of NUN 89007 PPS.

The disclosure also provides a cell culture of NUN 89007 PPS, and aplant regenerated from NUN 89007 PPS, which plant has all thecharacteristics of NUN 89007 PPS, when grown under the sameenvironmental conditions, as well as methods for regenerating NUN 89007PPS. Alternatively, a regenerated plant may have one characteristic thatis different from NUN 89007 PPS.

The disclosure further provides a vegetatively propagated plant ofvariety NUN 89007 PPS having all or all but one, two or three of themorphological and physiological characteristics NUN 89007 PPS, whengrown under the same environmental conditions.

The disclosure furthermore provides a pepper fruit produced on a plantgrown from a seed of NUN 89007 PPS.

In another aspect, the disclosure provides, a seed growing or grown on aplant of NUN 89007 PPS (i.e., produced after pollination of the flowerof NUN 89007 PPS).

DEFINITIONS

“Pepper” refers herein to plants of the species Capsicum annuum orfrutescens, and fruits thereof. The most commonly eaten part of a pepperis the fruit or berry. The fruit comprises a stem or peduncle orpedicel, calyx, placenta, fruit wall, veins, shoulder, base, apex,locule or lobe, septa, exocarp, mesocarp, endocarp, pericarp, optionallysecondary peppers, optionally capsaicin glands and optionally seed. Thestem or peduncle or pedicel, calyx, placenta, fruit wall, veins,shoulder, base, apex, locule or lobe, septa, exocarp, mesocarp,endocarp, pericarp, secondary peppers, capsaicin glands and seedcoat ofthe seed are maternal tissues, and thus they are genetically identicalto the plant on which they grow.

“Cultivated pepper” refers to plants of Capsicum annuum, or a closelyrelated species, e.g., varieties, breeding lines or cultivars of thespecies C. annuum as well as crossbreds thereof, or crossbreds withother Capsicum species, cultivated by humans and having good agronomiccharacteristics.

The terms “pepper plant designated NUN 89007 PPS”, “NUN 89007 PPS”, “NUN89007”, “NUN 89007 F1”, “89007 PPS” or “pepper 89007” are usedinterchangeably herein and refer to a pepper plant of variety NUN 89007PPS, representative seed of which has been deposited under AccessionNumber NCIMB 43268.

A “seed of NUN 89007 PPS” refers to a pepper seed which can be growninto a plant of NUN 89007 PPS, wherein a representative sample of viableseed of NUN 89007 PPS has been deposited under Accession Number NCIMB43268. A seed can be in any stage of maturity, for example a mature,viable seed, or an immature, non-viable seed. A seed comprises an embryoand maternal tissues.

An “embryo of NUN 89007 PPS” refers to an “F1 hybrid embryo” as presentin a seed of NUN 89007 PPS, a representative sample of said seed of NUN89007 PPS has been deposited under Accession Number NCIMB 43268.

A “seed grown on NUN 89007 PPS” refers to a seed grown on a mature plantof NUN 89007 PPS or inside a fruit of NUN 89007 PPS. The “seed grown onNUN 89007 PPS” contains tissues and DNA of the maternal parent, NUN89007 PPS. The “seed grown on NUN 89007 PPS” contains an F2 embryo. Whensaid seed is planted, it grows into a first generation progeny plant ofNUN 89007 PPS.

A “fruit of NUN 89007 PPS” refers to a fruit containing maternal tissuesof NUN 89007 PPS as deposited under Accession Number NCIMB 43268. In oneaspect, the fruit does not contain seed, i.e., the fruit isparthenocarpic. The skilled person is familiar with methods for inducingparthenocarpy. Those methods comprise chemically or genetically inducingparthenocarpy. Compounds suitable for chemically inducing parthenocarpyinclude auxins, gibberellins and cytokinins. Genetic parthenocarpy canbe induced by CαARF8 mutants (see, e.g., Tiwari et al., BMC PlantBiology 2011, 11:143 DOI: 10.1186/1471-2229-11-143 or U.S. Pat. No.8,492,619, which are herein incorporated by reference in theirentireties). A fruit can be in any stage of maturity for examplecomprising viable seed or comprising immature non-viable seed.

An “essentially homogeneous population of pepper seed” is a populationof seeds where at least 97%, 98%, 99% or more of the total population ofseed are seed of NUN 89007 PPS.

An “essentially homogeneous population of pepper plants” is a populationof plants where at least 97%, 98%, 99% or more of the total populationof plants are plants NUN 89007 PPS.

The phrase “essentially free from other seed” refers to a population ofseed where less than 3%, 2%, 1% or less of the total population of seedis seed that is not a pepper seed or, in another option, less than 3%,2%, 1% or less of the total population of seed is seed that is not seedof NUN 89007 PPS.

“Tissue culture” or “cell culture” refers to a composition comprisingisolated cells of the same or a different type or a collection of suchcells organized into parts of a plant. Tissue culture of various tissuesof pepper and regeneration of plants therefrom is well known and widelypublished (see, e.g., Sang-Gu et al. (1988), Plant Cell, Tissue andOrgan Culture 12: 67-74; Kothari et al., (2010) Biotechnology Advances28: 35-48). Similarly, the skilled person is well-aware how to prepare a“tissue culture” or “cell culture”.

“USDA descriptors” are the plant variety descriptors for pepper(Capsicum spp.) as published by the US Department of Agriculture,Agricultural Marketing Service, Plant Variety Protection Office,Beltsville, Md. 20705, and which can be downloaded from the world wideweb at ams.usda.gov under services/plant-variety-protection/pvpo-c-formsunder pepper. “Non-USDA descriptors” are other descriptors suitable fordescribing pepper.

“UPOV descriptors” are the plant variety descriptors described forpepper in the “Guidelines for the Conduct of Tests for Distinctness,Uniformity and Stability, TG/76/8 (Geneva 2006, revised 2015-03-25), aspublished by UPOV (International Union for the Protection of NewVarieties and Plants, and which can be downloaded from the world wideweb at upov.int under edocs/tgdocs/en/tg076.pdf and is hereinincorporated by reference in its entirety. Likewise, “UPOV methods” todetermine specific parameters for the characterization of pepper aredescribed at upov.int.

“RHS” refers to the Royal Horticultural Society of England whichpublishes an official botanical color chart quantitatively identifyingcolors according to a defined numbering system. The chart may bepurchased from Royal Horticulture Society Enterprise Ltd RHS Garden;Wisley, Woking; Surrey GU236QB, UK, e.g., the RHS color chart: 2007 (TheRoyal Horticultural Society, charity No: 222879, PO Box 313 LondonSW1P2PE).

“Reference Variety for NUN 89007 PPS” refers herein to variety ______, acommercial variety from company ______, which has been planted in atrial together with NUN 89007 PPS. USDA descriptors of NUN 89007 PPSwere compared to the USDA descriptors of ______.

“Harvest maturity” is referred to as the stage at which a pepper fruitis ripe or ready for harvest or the optimal time to harvest the fruitfor the market, for processing or for consumption. In one aspect,harvest maturity is the stage which allows proper completion of thenormal ripening.

“Flavor” refers to the sensory impression of a food or other substance,especially a pepper fruit or fruit part (fruit flesh) and is determinedmainly by the chemical senses of taste and smell. Flavor is influencedby texture properties and by volatile and/or non-volatile chemicalcomponents (organic acids, lipids, carbohydrates, salts, etc.).

“Plant part” includes any part of a plant, such as a plant organ (e.g.,harvested or non-harvested fruits), a plant cell, a plant protoplast, aplant cell tissue culture or a tissue culture from which a whole plantcan be regenerated, a plant cell that is intact in a plant, a clone, amicropropagation, plant callus, a plant cell clump, a plant transplant,a vegetative propagation, a seedling, a fruit, a harvested fruit, a partof a fruit, a leaf, a part of a leaf, pollen, an ovule, an embryo, apetiole, a shoot or a part thereof, a stem or a part thereof, a root ora part thereof, a root tip, a cutting, a seed, a part of a seed,hypocotyl, cotyledon, a scion, a graft, a stock, a rootstock, a pistil,an anther, and a flower or parts of any of these and the like. Seed canbe mature or immature. Pollen or ovules may be viable or non-viable.Also, any developmental stage is included, such as seedlings, cuttingsprior or after rooting, mature plants or leaves. Alternatively, a plantpart may also include a plant seed which comprises one or two sets ofchromosomes derived from the parent plant, e.g., from NUN 89007 PPS. AnF2 progeny produced from self-pollination of NUN 89007 PPS, will thuscomprise two sets of chromosomes derived from that variety, while an F2progeny derived from cross-fertilization of NUN 89007 PPS, will compriseonly one set of chromosomes from said variety and the other set ofchromosomes from the other parent.

“Harvested plant material” refers herein to plant parts (e.g., fruitsdetached from the whole plant), which have been collected for furtherstorage and/or further use.

“Yield” means the total weight of all pepper fruits harvested perhectare of a particular line or variety. It is understood that “yield”expressed as weight of all pepper fruits harvested per hectare can beobtained by multiplying the number of plants per hectare times the“yield per plant”.

“Marketable yield” means the total weight of all marketable pepperfruits, especially fruit that is not cracked, damaged or diseased,harvested per hectare of a particular line or variety. A “marketablefruit” is a fruit that has commercial value.

“Rootstock” or “stock” refers to the plant selected for its roots, inparticular for the resistance of the roots to diseases or stress (e.g.,heat, cold, salinity etc.). Normally, the quality of the fruit of theplant providing the rootstock is less important.

“Scion” refers to a part of the plant attached to the rootstock. Thisplant is selected for its stems, leaves, flowers, or fruits. The scioncontains the desired genes to be duplicated in future production by thestock/scion plant and may produce the desired pepper fruit.

“Stock/scion” or grafted plant refers to a pepper plant comprising arootstock from one plant grafted to a scion from another plant.

A plant having “all the physiological and morphological characteristics”of a referred-to-plant means a plant showing the physiological andmorphological characteristics of the referred-to-plant when grown underthe same environmental conditions, preferably in the same experiment;the referred-to-plant can be a plant from which it was derived, e.g.,the progenitor plant, the parent, the recurrent parent, the plant usedfor tissue- or cell culture, etc. A physiological or morphologicalcharacteristic can be a numerical characteristic or a non-numericalcharacteristic. In one aspect, a plant has “all but one, two or three ofthe physiological and morphological characteristics” of areferred-to-plant, or “all the physiological and morphologicalcharacteristics” of Table 1 and/or 2 and/or 3 or “all or all but one,two or three of the physiological and morphological characteristics” ofTable 1 and/or 2 and/or 3.

The physiological and/or morphological characteristics mentioned aboveare commonly evaluated at significance levels of 1%, 5% or 10% if theyare numerical, or for having an identical degree (or type) if notnumerical, if measured under the same environmental conditions. Forexample, a progeny plant or a Single Locus Converted plant or a mutatedplant of NUN 89007 PPS, may have one or more (or all) of thephysiological and/or morphological characteristics of said varietylisted in Table 1 and/or 2 and/or 3, as determined at the 5%significance level (i.e., p<0.05) when grown under the sameenvironmental conditions.

“Distinguishing characteristics” or “distinguishing morphological and/orphysiological characteristics” refers herein to the characteristicswhich distinguish (i.e., are different) between the new variety andother pepper varieties, such as the Reference Variety, when grown underthe same environmental conditions. The distinguishing characteristicsbetween NUN 89007 PPS and the Reference Variety are described herein andalso can be seen in Table 1 and/or Table 2 and/or 3. When comparing NUN89007 PPS with different varieties, the distinguishing characteristicswill be different. In one aspect, the distinguishing characteristics maytherefore include at least one, two, three or more (or all) of thecharacteristics listed in Table 1 and/or 2 and/or 3. All numericaldistinguishing characteristics are statistically significantly differentat p <0.05 between NUN 89007 PPS and the other variety, e.g., theReference Variety.

NUN 89007 PPS has the following distinguishing characteristics whencompared to the Reference Variety as shown in Table 1 and/or 2: 1)______; 2) ______; 3) ______; 4) ______; 5) ______; 6) ______; 7)______; 8) ______; 9) ______; and/or 10) ______. This can be seen inTable 1 and/or 2, where the USDA characteristics of NUN 89007 PPS arecompared to the characteristics of Reference Variety, when grown underthe same environmental conditions.

Thus, a pepper plant “comprising the distinguishing characteristics ofNUN 89007 PPS” (such as a progeny plant) refers herein to a plant whichdoes not differ significantly from said variety in the distinguishingcharacteristics above. Therefore, in one aspect, a plant (such as aprogeny plant of NUN 89007 PPS) is provided which does not differsignificantly from NUN 89007 PPS in the distinguishing characteristicsabove.

Similarity and differences between two different plant lines orvarieties can be determined by comparing the number of morphologicaland/or physiological characteristics (e.g. the characteristics as listedin Table 1 and/or 2 and/or 3) that are the same (i.e., statistically notsignificantly different) or that are different (i.e., statisticallysignificantly different) between the two plant lines or varieties whengrown under the same environmental conditions. A numericalcharacteristic is considered to be “the same” when the value for anumeric characteristic is not significantly different at the 1% (p<0.01)or 5% (p<0.05) significance level, using one way Analysis of variance(ANOVA), a standard method known to the skilled person. Non-numerical or“degree” or “type” characteristic is considered “the same” when thevalues have the same “degree” or “type” when scored using USDA and/orUPOV descriptors, if the plants are grown under the same environmentalconditions.

As used herein, the term “variety”, “cultivated pepper” or “cultivar”means a plant grouping within a single botanical taxon of the lowestknown rank, which grouping, irrespective of whether the conditions forthe grant of a breeder's right are fully met, can be defined by theexpression of the characteristics resulting from a given genotype orcombination of genotypes, distinguished from any other plant grouping bythe expression of at least one of the said characteristics andconsidered as a unit with regard to its suitability for being propagatedunchanged.

A “plant line” is for example a breeding line which can be used todevelop one or more varieties. A breeding line is typically highlyhomozygous.

“Hybrid variety” or “F1 hybrid” refers to the seeds harvested fromcrossing two inbred (nearly homozygous) parental lines. For example, thefemale parent is pollinated with pollen of the male parent to producehybrid (F1) seeds on the female parent.

“Regeneration” refers to the development of a plant from cell culture ortissue culture or vegetative propagation.

“Vegetative propagation”, “vegetative reproduction” or “clonalpropagation” are used interchangeably herein and mean a method of takinga part of a plant and allowing that plant part to form at least roots,and also refer to the plant or plantlet obtained by that method.Optionally, the vegetative propagation is grown into a mature plant. Theskilled person is aware of what plant parts are suitable for use in themethod.

“Selfing” refers to self-pollination of a plant, i.e., the transfer ofpollen from the anther to the stigma of the same plant.

“Crossing” refers to the mating of two parent plants. The termencompasses “cross-pollination” and “selfing”.

“Cross-pollination” refers to the fertilization by the union of twogametes from different plants.

As used herein, the terms “resistance” and “tolerance” are usedinterchangeably to describe plants that show no symptoms orsignificantly reduced symptoms to a specified biotic pest, pathogen,abiotic influence or environmental condition compared to a susceptibleplant. These terms are optionally also used to describe plants showingsome symptoms but that are still able to produce marketable product withan acceptable yield.

The term “traditional breeding techniques” encompasses herein crossing,selfing, selection, doubled haploid production, embryo rescue,protoplast fusion, marker assisted selection, mutation breeding, etc. asknown to the breeder (i.e., methods other than geneticmodification/transformation/transgenic methods), by which, for example,a genetically heritable trait can be transferred from one pepper line orvariety to another.

“Backcrossing” is a traditional breeding technique used to introduce atrait into a plant line or variety. The plant containing the trait iscalled the donor plant and the plant into which the trait is transferredis called the recurrent parent. An initial cross is made between thedonor parent and the recurrent parent to produce a progeny plant.Progeny plants which have the trait are then crossed to the recurrentparent. After several generations of backcrossing and/or selfing therecurrent parent comprises the trait of the donor. The plant generatedin this way may be referred to as a “single trait converted plant”. Thetechnique can also be used on a parental line of a hybrid.

“Progeny” as used herein refers to a plant obtained from a plantdesignated NUN 89007 PPS. A progeny may be obtained by regeneration ofcell culture or tissue culture or parts of a plant of said variety orselfing of a plant of said variety or by producing seeds of a plant ofsaid variety. In further aspects, progeny may also encompass plantsobtained from crossing of at least one plant of said variety withanother pepper plant of the same variety or another variety or(breeding) line, or with wild pepper plants. A progeny may comprise amutation or a transgene. A “first generation progeny” or is the progenydirectly derived from, obtained from, obtainable from or derivable fromthe parent plant by, e.g., traditional breeding methods (selfing and/orcross-pollinating) or regeneration (optionally combined withtransformation and mutation). Thus, a plant of NUN 89007 PPS, is themale parent, the female parent or both of a first generation progeny ofthat variety. Progeny may have all the physiological and morphologicalcharacteristics of NUN 89007 PPS, when grown under the sameenvironmental conditions and/or progeny may have (be selected forhaving) one or more of the distinguishing characteristics of pepper ofthe disclosure. Using common breeding methods such as backcrossing orrecurrent selection, mutation or transformation, one or more specificcharacteristics may be introduced into said variety, to provide or aplant comprising all but 1, 2, or 3 or more of the morphological andphysiological characteristics of NUN 89007 PPS (as listed in Table 1and/or 2 and/or 3).

The terms “gene converted” or “conversion plant” or “single locusconverted plant” in this context refer to pepper plants which aredeveloped by traditional breeding techniques e.g., backcrossing or viagenetic engineering or through mutation breeding, wherein essentiallyall of the desired morphological and physiological characteristics ofthe parent variety or line are recovered, in addition to the one or moregenes transferred into the parent via e.g., backcrossing technique(optionally including reverse breeding or reverse synthesis of breedinglines). It is understood that only the addition of a furthercharacteristic (e.g., addition of gene conferring a furthercharacteristic, such as a disease resistance gene), but also thereplacement/modification of an existing characteristic by a differentcharacteristic is encompassed herein.

Likewise, a “Single Locus Converted (Conversion) Plant” refers to aplant developed by plant breeding techniques comprising or consisting ofmutation and/or by genetic transformation and/or by traditional breedingtechniques, such as backcrossing, wherein essentially all of the desiredmorphological and physiological characteristics of a pepper variety arerecovered in addition to the characteristics of the single locus havingbeen transferred into the variety via the backcrossing technique. Incase of a hybrid, the gene may be introduced in the male or femaleparental line.

“Average” refers herein to the arithmetic mean.

The term “mean” refers to the arithmetic mean of several measurements.The skilled person understands that the appearance of a plant depends tosome extent on the growing conditions of said plant. Thus, the skilledperson will know typical growing conditions for NUN 89007 PPS. The mean,if not indicated otherwise within this application, refers to thearithmetic mean of measurements on at least 10 different, randomlyselected plants of a variety or line.

DETAILED DESCRIPTION OF VARIOUS ASPECTS OF THE DISCLOSURE

The disclosure relates to a plant of NUN 89007 PPS, wherein arepresentative sample of seeds of said variety has been deposited underthe Budapest Treaty, with Accession number NCIMB 43268. NUN 89007 PPS isorange, Multi Sweet Pointed sweet pepper for the snacking segment. It issuitable for early harvest with concentrated fruit set.

The disclosure also relates to a seed of pepper variety, referred to asNUN 89007 PPS, wherein a representative sample of said seed has beendeposited under the Budapest Treaty, with Accession number NCIMB 43268.

In another aspect, the disclosure provides for a pepper plant part ofvariety NUN 89007 PPS, preferably a fruit, a representative sample ofseed from said variety has been deposited under the Budapest Treaty,with Accession number NCIMB 43268.

A seed of hybrid variety NUN 89007 PPS is obtainable by crossing themale parent of said variety with the female parent of said variety andharvesting the seeds produced on the female parent. The resultant seedsof said variety can be grown to produce plants of said variety. In oneaspect, a seed or a plurality of seeds of said variety are packaged intoa container of any size or type (e.g., bags, cartons, cans, etc.). Theseed may be disinfected, primed and/or treated with various compounds,such as seed coatings or crop protection compounds. The seed produces aplant of NUN 89007 PPS.

Also provided is a plant of pepper variety NUN 89007 PPS, or a fruit orother plant part thereof, produced from a seed, wherein a representativesample of said seeds has been deposited under the Budapest Treaty, withAccession Number NCIMB 43268.

Also a plant part obtained from variety NUN 89007 PPS, is provided,wherein said plant part is: a fruit, a harvested fruit, a part of afruit, a leaf, a part of a leaf, pollen, an ovule, a cell, a petiole, ashoot or a part thereof, a stem or a part thereof, a root or a partthereof, a root tip, a cutting, a seed, a part of a seed, seed coat oranother maternal tissue which is part of a seed grown on said varieties,hypocotyl, cotyledon, a scion, a stock, a rootstock, a pistil, ananther, and a flower or a part thereof. Fruits are particularlyimportant plant parts. Fruits may be parthenocarpic, or seedless, orcontain immature and/or nonviable seeds. In a further aspect, the plantpart obtained from variety NUN 89007 PPS is a cell, optionally a cell ina cell or tissue culture. That cell may be grown into a plant of NUN89007 PPS. A part of a variety of NUN 89007 PPS (or of progeny NUN 89007PPS or of a plant having all physiological and/or morphologicalcharacteristics but one, two or three which are different from those ofNUN 89007 PPS) further encompasses any cells, tissues, organs obtainablefrom the seedlings or plants in any stage of maturity.

The disclosure also provides for a food or feed product or a processedproduct comprising or consisting of a plant part described herein.Preferably, the plant part is a pepper fruit or part thereof and/or anextract from a fruit or another plant part described herein comprisingat least one cell of NUN 89007 PPS. The food or feed product may befresh or processed, e.g., dried, grinded, powdered, pickled, chopped,cooked, roasted, in a sauce, in a sandwich, pasted, puréed orconcentrated, juiced, pickled, canned, steamed, boiled, fried, blanchedand/or frozen, etc.

Such a plant part of NUN 89007 PPS can be stored and/or processedfurther. The disclosure thus also provides for a food or feed productcomprising one or more of such parts, such as canned, chopped, cooked,roasted, in a sauce, in a sandwich, pasted, puréed or concentrated,juiced, frozen, dried, pickled, or powdered pepper fruit from saidvariety or from progeny of said variety, or from a plant having all butone, two or three physiological and/or morphological characteristics ofNUN 89007 PPS.

In another aspect, the disclosure provides for a pepper fruit of varietyNUN 89007 PPS, or a part of a fruit of said variety. The fruit can be inany stage of maturity, for example, immature or mature. In anotheraspect, the disclosure provides for a container comprising or consistingof a plurality of harvested pepper fruits or parts of fruits of saidvariety, or fruits of progeny thereof, or fruits of a derived variety.

In another aspect, the plant, plant part or seed of NUN 89007 PPS isinside a container, for example, containers such as cans, boxes, crates,bags, cartons, Modified Atmosphere Packaging, films (e.g., biodegradablefilms), etc. comprising a plant or a part of a plant (fresh and/orprocessed) or a seed of NUN 89007 PPS. In a particular aspect, thecontainer comprises a plurality of seeds, or a plurality of plant partsof NUN 89007 PPS or.

The disclosure further relates to a pepper variety, referred to as NUN89007 PPS, which when compared to its Reference Variety ______, has thefollowing distinguishing characteristics as shown in Table 1 and/or2: 1) ______; 2) ______; 3) ______; 4) ______; 5) ______; 6) ______; 7)______; 8) ______; 9) ______; and/or 10) ______, where thecharacteristics are determined at the 5% significance level for plantsgrown under the same environmental conditions. Also encompassed by thepresent disclosure are parts of that plant.

In one aspect, a plant of NUN 89007 PPS or a progeny plant thereof,comprises all of the following morphological and/or physiologicalcharacteristics (i.e., average values of distinguishing characteristics,as indicated on the USDA Objective description of variety—pepper (unlessindicated otherwise)) as shown in Table 1 and/or 2: 1) ______; 2)______; 3) ______; 4) ______; 5) ______; 6) ______; 7) ______; 8)______; 9) ______; and 10) ______, where the characteristics aredetermined at the 5% significance level for plants grown under the sameenvironmental conditions. An example of values for the distinguishingcharacteristics collected in a trial run according to UDSA requirementscan be found in Table 1 and/or 2. A part of this plant is also provided.

NUN 89007 PPS may further exhibit one or more of the following traits:a) ______, b) ______, c) ______, and/or d) ______.

In another aspect, NUN 89007 PPS comprises resistance to Pepper MildMottle Virus (PMMoV) Pathotype 0 and/or to Potato Y Virus Pathotype 0and Pathotype 1, measured according to UPOV standards described inTG/76/8.

The disclosure further provides a pepper plant which does not differfrom the physiological and morphological characteristics of the plant ofNUN 89007 PPS as determined at the 1%, 2%, 3%, 4% or 5% significancelevel when grown under the same environmental conditions. In aparticular aspect, the plants are measured in the same trial (e.g., thetrial is conducted as recommended by the USDA or UPOV). The disclosurealso comprises a part of said plant.

The disclosure also provides a tissue or cell culture comprising cellsof NUN 89007 PPS. Such a tissue culture can, for example, be grown onplates or in liquid culture, or be frozen for long term storage. Thecells of NUN 89007 PPS used to start the culture can be selected fromany plant part suitable for vegetative reproduction, or in a particularaspect can be one or more of an embryo, meristem, a cotyledon, ahypocotyl, pollen, a leaf, an anther, a root, a root tip, a pistil, apetiole, a flower, a fruit, seed, and/or a stem. In another particularaspect, the tissue culture does not contain somaclonal variation or hasreduced somaclonal variation. The skilled person is familiar withmethods to reduce or prevent somaclonal variation, including regularreinitiation.

In one aspect, the disclosure provides a pepper plant regenerated fromthe tissue or cell culture of NUN 89007 PPS, wherein the regeneratedplant is not significantly different from NUN 89007 PPS in all, or allbut one, two or three, of the physiological and morphologicalcharacteristics (determined at the 5% significance level when grownunder the same environmental conditions). Optionally, the plant has one,two or three the physiological and morphological characteristics thatare affected by a mutation or by transformation. In another aspect, thedisclosure provides a pepper plant regenerated from the tissue or cellculture of NUN 89007 PPS, wherein the plant has all of the physiologicaland morphological characteristics of said variety determined at the 5%significance level when grown under the same environmental conditions.In these cases, similarity or difference of a characteristic isdetermined by measuring the characteristics of a representative numberof plants grown under the same environmental conditions, determiningwhether type/degree characteristics are the same and determining whethernumerical characteristics are different at the 5% significance level.

NUN 89007 PPS, or its progeny, or a plant having all physiologicaland/or morphological characteristics but one, two or three which aredifferent from those of NUN 89007 PPS, can also be reproduced usingvegetative reproduction methods. Therefore, the disclosure provides fora method of producing a plant, or a part thereof, of variety NUN 89007PPS, comprising vegetative propagation of said variety. Vegetativepropagation comprises regenerating a whole plant from a plant part ofvariety NUN 89007 PPS (or from a progeny of said variety or from or aplant having all physiological and/or morphological characteristics ofsaid variety but one, two or three different characteristics), such as acutting, a cell culture or a tissue culture.

The disclosure also concerns methods of vegetatively propagating a partof the plant of the disclosure NUN 89007 PPS. In certain aspects, themethod comprises the steps of: (a) collecting tissue or cells capable ofbeing propagated from a plant described herein; (b) cultivating saidtissue or cells to obtain proliferated shoots; and (c) rooting saidproliferated shoots, to obtain rooted plantlets. Steps (b) and (c) mayalso be reversed, i.e. first cultivating said tissue to obtain roots andthen cultivating the tissue to obtain shoots, thereby obtaining rootedplantlets. The rooted plantlets may then be further grown, to obtainplants. In one aspect, the method further comprises step (d) growingplants from said rooted plantlets. Therefore, the method also comprisesregenerating a whole plant from said part of NUN 89007 PPS. In aparticular aspect, the part of the plant to be propagated is a cutting,a cell culture or a tissue culture.

The disclosure also provides for a vegetatively propagated plant ofvariety NUN 89007 PPS (or from progeny of said variety or from or aplant having all but one, two or three physiological and/ormorphological characteristics of said variety), wherein the plant hasall of the morphological and physiological characteristics of NUN 89007PPS, when the characteristics are determined at the 5% significancelevel for plants grown under the same conditions. In another aspect, thepropagated plant has all but one, two or three of the morphological andphysiological characteristics of NUN 89007 PPS, when the characteristicsare determined at the 5% significance level for plants grown under thesame conditions. A part of said propagated plant or said propagatedplant with one, two or three differences is also included.

In another aspect, the disclosure provides a method for producing apepper plant part, preferably a fruit, comprising growing a plant of NUN89007 PPS until it sets at least one fruit, and collecting the fruit.Preferably, the fruit is collected at harvest maturity. In anotheraspect, the fruit is collected when the seed is ripe. A plant of NUN89007 PPS can be produced by seeding directly in the soil (e.g., field)or by germinating the seeds in controlled environment conditions (e.g.,greenhouses) and optionally then transplanting the seedlings into thefield. For example, the seed can be sown into prepared seed beds wherethey will remain for the entire production the crop (see, e.g.,https://anrcatalog.ucanr.edu/pdf/7217.pdf). Stakes and plastic mulchesmay also be used for peppers for the fresh the market, particularly,when peppers are to be harvested at mature fruit color and to promoteearliness and yield. On the other hand, no stake or mulch is used forprocessing peppers. Moreover, pepper can also be grown entirely ingreenhouses or tunnels (see, e.g.,https://www.wifss.ucdavis.edu/wp-content/uploads/2016/10/Peppers_PDF.pdf).

In still another aspect, the disclosure provides a method of producing apepper plant, comprising crossing a plant of pepper NUN 89007 PPS with asecond pepper plant at least once, allowing seed to develop andoptionally harvesting said progeny seed. The skilled person can selectprogeny from said crossing. Optionally, the progeny (grown from theprogeny seed) is crossed twice, thrice, or four, five, six or seventimes, and allowed to set seed. In one aspect of the disclosure, thefirst “crossing” further comprises planting seeds of a first and asecond parent pepper plant, often in proximity so that pollination willoccur; for example, mediated by insect vectors. Alternatively, pollencan be transferred manually. Where the plant is self-pollinated,pollination may occur without the need for direct human interventionother than plant cultivation. After pollination the plant can produceseed.

In yet another aspect, the disclosure provides a method of producing aplant, comprising selfing a plant of variety NUN 89007 PPS one or moretimes, and selecting a progeny plant from said selfing. In one aspect,the progeny plant retains all the distinguishing characteristics of NUN89007 PPS when grown under the same environmental conditions. In adifferent aspect the progeny plant comprises all (or all but one, two orthree) of the physiological and morphological characteristic of NUN89007 PPS of Table 1, and/or 2 and/or 3.

In other aspects, the disclosure provides a progeny plant of variety NUN89007 PPS, such as a progeny plant obtained by further breeding thatvariety. Further breeding with the variety includes selfing that varietyone or more times and/or cross-pollinating that variety with anotherpepper plant or variety one or more times. In particular, the disclosureprovides for a progeny plant that retains all the morphological andphysiological characteristics of NUN 89007 PPS or, in another aspect, aprogeny plant that retains all, or all but one, two or three, of themorphological and physiological characteristics of NUN 89007 PPS,optionally all or all but one, two or three of the characteristics aslisted in Table 1 and/or 2 and/or 3, when grown under the sameenvironmental conditions, determined at the 5% significance level fornumerical characteristics. In a particular aspect, the progeny is afirst generation progeny, i.e., the ovule or the pollen (or both) usedin the crossing is an ovule or pollen of variety NUN 89007 PPS, wherethe pollen comes from an anther of and the ovule comes from an ovary ofNUN 89007 PPS. In another aspect, the disclosure provides for avegetative reproduction of the variety and a plant having all, or allbut 1, 2, or 3 of the physiological and morphological characteristics ofNUN 89007 PPS (e.g., as listed in Table 1 and/or 2 and/or 3).

The disclosure also provides a method for collecting pollen comprisingcollecting pollen from a plant of NUN 89007 PPS. Alternatively, themethod comprises growing a plant of NUN 89007 PPS until at least oneflower contains pollen and collecting the pollen. In particular aspect,the pollen is collected when it is mature or ripe. A suitable method forcollecting pollen comprises collecting anthers or the part of the antherthat contains pollen, for example by cutting the anther or the part ofthe anther off. Pollen can be collected in a container. Optionally,collected pollen can be used to pollinate a pepper flower.

The morphological and/or physiological differences between two differentindividual plants of the disclosure (e.g., between NUN 89007 PPS, and aprogeny thereof) or between a plant of NUN 89007 PPS or progeny of saidvariety, or a plant having all, or all but 1, 2, or 3, of thephysiological and morphological characteristics of NUN 89007 PPS (orall, or all but 1, 2, or 3 of the characteristics as listed in Table 1and/or 2 and/or 3) and another known variety can easily be establishedby growing said variety next to each other or next to the other variety(in the same field, under the same environmental conditions), preferablyin several locations which are suitable for said pepper cultivation, andmeasuring morphological and/or physiological characteristics of a numberof plants (e.g., to calculate an average value and to determine thevariation range/uniformity within the variety). For example, trials canbe carried out in Acampo Calif., USA (N 38 degrees 07′ 261″/W 121degrees 18′ 807″, USA), whereby various characteristics, for examplematurity, days from seeding to harvest, plant habit, plant attitude,leaf shape, leaf color, blistering, numbers of flowers per leaf axil,number of calyx lobes, number of petals, fruit group, immature fruitcolor, mature fruit color, pungency, flavor, fruit glossiness, fruitsize, fruit shape, average number of fruits per plant, seed size, seedweight, anthocyanin level, disease resistance, insect resistance, can bemeasured and directly compared for species of pepper. Thus, thedisclosure comprises pepper plant having one, two or three physiologicaland/or morphological characteristics which are different from those ofthe plant of NUN 89007 PPS and which otherwise has all the physiologicaland morphological characteristics of said variety, when determined atthe 5% significance level for plants grown under the same environmentalconditions. In a particular aspect, the different characteristic(s)is/are result of breeding with NUN 89007 PPS and selection of progenyplant comprising 1, 2, or 3 characteristics which are different than inNUN 89007 PPS. In another aspect, the different characteristic is theresult of a mutation (e.g., spontaneous mutation or a human inducedmutation through e.g., targeted mutagenesis or traditional mutagenesissuch as chemically or radiation induced mutagenesis), or it is theresult of transformation.

The morphological and physiological characteristics (and thedistinguishing characteristics) of NUN 89007 PPS are provided in theExamples, in Table 1 and/or 2 and/or 3. Encompassed herein is also aplant obtainable from NUN 89007 PPS (e.g., by selfing and/or crossingand/or backcrossing with said variety and/or progeny of said variety)comprising all or all but one, two or three of the physiological andmorphological characteristics of NUN 89007 PPS listed in Table 1 and/or2 and/or 3 as determined at the 5% significance level for numericalcharacteristics or identical for non-numerical characteristics whengrown under the same environmental conditions and/or comprising one ormore (or all; or all except one, two or three) characteristics whengrown under the same environmental conditions. The morphological and/orphysiological characteristics may vary somewhat with variation in theenvironment (such as temperature, light intensity, day length, humidity,soil, fertilizer use), which is why a comparison under the sameenvironmental conditions is preferred. Colors can best be measured usingthe Royal Horticultural Society Chart.

In yet a further aspect, the disclosure provides for a method ofproducing a pepper plant. The method comprises crossing a plant of NUN89007 PPS, or a plant comprising all but 1, 2, or 3 of the morphologicaland physiological characteristics of said variety (as listed in Table 1and/or 2 and/or 3), or a progeny plant thereof, either as male or asfemale parent, with a second pepper plant (or a wild relative of pepper)one or more times, and/or selfing a pepper plant of NUN 89007 PPS or aprogeny plant thereof, one or more times, and selecting progeny fromsaid crossing and/or selfing. The second pepper plant may, for example,be a line or variety of the species species Capsicum annuum, C.frutecens, C. baccatum, C. chinense, or other Capsicum species.

The disclosure provides for methods of producing plants which retain allthe morphological and physiological characteristics of a plant of NUN89007 PPS. The disclosure provides also for methods of producing a plantcomprising all but 1, 2, or 3 or more of the morphological andphysiological characteristics of NUN 89007 PPS (e.g., as listed in Table1 and/or 2 and/or 3), but which are still genetically closely related tosaid variety. The relatedness can, for example, be determined byfingerprinting techniques (e.g., making use of isozyme markers and/ormolecular markers such as Single-nucleotide polymorphism (SNP) markers,amplified fragment length polymorphism (AFLP) markers, microsatellites,minisatellites, Random Amplified Polymorphic DNA (RAPD) markers,restriction fragment length polymorphism (RFLP) markers and others). Aplant is “closely related” to NUN 89007 PPS if its DNA fingerprint is atleast 80%, 90%, 95% or 98% identical to the fingerprint of said variety.In a particular aspect, AFLP markers are used for DNA fingerprinting(see, e.g., Vos et al. 1995, Nucleic Acid Research 23: 4407-4414). Aclosely related plant may have a Jaccard's Similarity index of at leastabout 0.8, preferably at least about 0.9, 0.95, 0.98 or more (see, e.g.,Ince, et. al., 2010, Biochem Genet 48:83-95). The disclosure alsoprovides a plant and a variety obtained or selected by applying thesemethods on NUN 89007 PPS. Such a plant may be produced by crossingand/or selfing, or alternatively, a plant may simply be identified andselected amongst plants of said variety, or progeny of said variety,e.g., by identifying a variant within NUN 89007 PPS, or within progenyof said variety (e.g., produced by selfing) which variant differs fromsaid variety in one, two or three of the morphological and/orphysiological characteristics (e.g., in one, two or three distinguishingcharacteristics), e.g., those listed in Table 1 and/or 2 and/or 3 orothers. In one aspect, the disclosure provides a pepper plant having aJaccard's Similarity index with NUN 89007 PPS of at least 0.8, e.g., atleast 0.85, 0.9, 0.95, 0.98 or even at least 0.99.

In some aspects, the disclosure provides a pepper plant comprisinggenomic DNA having at least 95%, 96%, 97%, 98% or 99% sequence identitycompared to the genomic DNA sequence of a plant of NUN 89007 PPS asdeposited under Accession Number NCIMB 43268. In some aspects, thepepper plant further comprises all or all but 1, 2, or 3 of themorphological and physiological characteristics of NUN 89007 PPS (e.g.,as listed in Table 1 and/or 2). In other aspects, the pepper plant is ahybrid derived from a seed or plant of NUN 89007 PPS. In other aspects,the pepper plant further comprises all of the distinguishingcharacteristics of a plant of NUN 89007 PPS.

For the purpose of this disclosure, the “sequence identity” ofnucleotide sequences, expressed as a percentage, refers to the number ofpositions in the two optimally aligned sequences which have identicalresidues (×100) divided by the number of positions compared. A gap,i.e., a position in the pairwise alignment where a residue is present inone sequence but not in the other, is regarded as a position withnon-identical residues. A pairwise global sequence alignment of twonucleotide sequences is found by aligning the two sequences over theentire length according to the Needleman and Wunsch global alignmentalgorithm described in Needleman and Wunsch, 1970, J. Mol. Biol.48(3):443-53). A full implementation of the Needleman-Wunsch globalalignment algorithm is found in the needle program in The EuropeanMolecular Biology Open Software Suite (see, e.g., EMBOSS, Rice et al.,Trends in Genetics June 2000, vol. 16, No. 6. pp. 276-277).

The disclosure also provides methods for determining the identity ofparental lines of plants described herein, in particular the identity ofthe female line. US2015/0126380, which is hereby incorporated byreference, relates to a non-destructive method for analyzing maternalDNA of a seed. In this method, the DNA is dislodged from the seed coatsurface and can be used to collect information on the genome of thematernal parent of the seed. This method for analyzing maternal DNA of aseed comprises contacting a seed with a fluid to dislodge DNA from theseed coat surface, and analyzing the DNA thus dislodged from the seedcoat surface using methods known in the art. The skilled person is thusable to determine whether a seed has grown on a plant of a plant of NUN89007 PPS, is a progeny of said variety, because the seed coat of theseed is a maternal tissue genetically identical to said variety. In oneaspect, the disclosure relates to a seed coat comprising maternal tissueof NUN 89007 PPS. In another aspect, the disclosure relates to a pepperseed comprising a maternal tissue of NUN 89007 PPS. In anotherparticular aspect, the disclosure provides for a method of identifyingthe female parental line of NUN 89007 PPS by analyzing the seed coat ofa seed of that variety. In another aspect, the skilled person candetermine whether a seed is grown on NUN 89007 PPS by analysing the seedcoat of said seed.

By crossing and/or selfing (one or more) single traits may be introducedinto NUN 89007 PPS (e.g., using backcrossing breeding schemes), whileretaining the remaining morphological and physiological characteristicsof said variety and/or while retaining one or more or all distinguishingcharacteristics. A single trait converted plant may thereby be produced.For example, disease resistance genes may be introduced, genesresponsible for one or more quality traits, yield, etc. Both singlegenes (e.g., dominant or recessive) and one or more QTLs (quantitativetrait loci) may be transferred into NUN 89007 PPS by breeding with saidvariety.

Alternatively, a single trait converted plant or single locus convertedplant of NUN 89007 PPS may be produced by (i) genetically transformingor mutating cells of NUN 89007 PPS; (ii) growing the cells into a plant;and (iii) optionally selecting a plant that contains the desired singlelocus conversion. The skilled person is familiar with various techniquesfor genetically transforming a single locus in a plant cell, or mutatingsaid cells.

Any pest or disease resistance genes may be introduced into a plant ofNUN 89007 PPS, progeny of said variety or into a plant comprising allbut 1, 2, or 3 or more of the morphological and physiologicalcharacteristics of NUN 89007 PPS (e.g., as listed in Table 1 and/or 2and/or 3). Resistance to one or more of the following diseases or pestsmay be introduced into plants of the disclosure: Cucumber Mosaic Virus,Curly Top Virus, Pepper Mild Mottle Virus, Potato Y Virus, Tobacco EtchVirus, Tobacco Mosaic Virus, Tomato Spotted Wilt Virus, Anthracnose(Gloeosporium piperatum), Bacterial Spot (Xanthomonas vesicatoria),Cercospora Leaf Spot (Cercospora capsici), Nematode (Meloidogyneincognita acrita), Phytophthora Root Rot (Phytophthora capsici), RipeRot (Vermicularia capsici), Southern Blight (Sclerotium rolfsii) and/orVerticillium Wilt (Verticillium dahliae). Other resistance genes,against pathogenic viruses, fungi, bacteria, nematodes, insects or otherpests may also be introduced. In another aspect, the resistance is TSWVresistance.

The disclosure also provides a method for developing a pepper plant in apepper breeding program, using a pepper plant described herein, or itsparts as a source of plant breeding material. Suitable plant breedingtechniques are recurrent selection, backcrossing, pedigree breeding,mass selection, mutation breeding and/or genetic marker enhancedselection. For example, in one aspect, the method comprises crossing NUN89007 PPS or progeny of said variety, or a plant comprising all but 1,2, or 3 or more of the morphological and physiological characteristicsof NUN 89007 PPS (e.g., as listed in Table 1 and/or 2 and/or 3), with adifferent pepper plant, and wherein one or more offspring of thecrossing are subject to one or more plant breeding techniques: recurrentselection, backcrossing, pedigree breeding, mass selection, mutationbreeding and genetic marker enhanced selection (see e.g., Martin et al.2008, Australian Journal of Crop Science 1(2): 43-46). For breedingmethods in general (see, e.g., Principles of Plant Genetics andBreeding, 2007, George Acquaah, Blackwell Publishing, ISBN-13:978-1-4051-3646-4).

The disclosure also provides a pepper plant comprising at least a firstset of the chromosomes of pepper variety NUN 89007 PPS, a sample of seedof said variety is deposited under Accession Number NCIMB 43268;optionally further comprising a single locus conversion or a mutation,wherein said plant has all of the morphological and physiologicalcharacteristics of the plant comprising at least a first set of thechromosomes of said variety. In another aspect, this single locusconversion confers a trait: yield, compact pepper, fruit quality,storage properties, color, male sterility, herbicide tolerance, insectresistance, pest resistance, disease resistance, environmental stresstolerance, modified carbohydrate metabolism and modified proteinmetabolism or ripening.

In one aspect, a plant of NUN 89007 PPS may also be mutated (by e.g.,irradiation, chemical mutagenesis, heat treatment, etc.) and mutatedseeds or plants may be selected in order to change one or morecharacteristics of said variety. Methods such as TILLING may be appliedto pepper populations in order to identify mutants. Similarly, NUN 89007PPS may be transformed and regenerated, whereby one or more chimericgenes are introduced into the variety or into a plant comprising all but1, 2, 3, or more of the morphological and physiological characteristics(e.g., as listed in Table 1 and/or 2 and/or 3). Transformation can becarried out using standard methods, such as Agrobacterium tumefaciensmediated transformation or biolistics, followed by selection of thetransformed cells and regeneration into plants. A desired trait (e.g.,gene(s) conferring pest or disease resistance, herbicide, fungicide orinsecticide tolerance, etc.) can be introduced into NUN 89007 PPS, orprogeny of said variety, by transforming said variety or progeny of saidvariety with a transgene that confers the desired trait, wherein thetransformed plant retains all or all but one, two or three of thephenotypic and/or morphological and/or physiological characteristics ofNUN 89007 PPS or the progeny of said variety and contains the desiredtrait.

The disclosure also provides a plant or a cell of a plant comprising adesired trait produced by mutating a plant of variety NUN 89007 PPS or acell thereof and selecting a plant the desired trait, wherein themutated plant retains all or all but one of the phenotypic andmorphological characteristics of said variety, optionally as describedfor each variety in in Table 1 and/or 2 and/or 3, and contains thedesired trait and wherein a representative sample of seed of variety NUN89007 PPS is deposited under Accession Number NCIMB 43268. In a furtheraspect, the desired trait is: yield, compact pepper, fruit quality, malesterility, herbicide tolerance, insect resistance, pest resistance,disease resistance, Powdery mildew resistance without necrosis,environmental stress tolerance, modified carbohydrate metabolism,modified protein metabolism or ripening.

In one aspect, the disclosure provides a method for inducing mutation inNUN 89007 PPS, comprising:

-   -   a) exposing a seed, a plant or a plant part or a cell of NUN        89007 PPS to a mutagenic compound or to radiation, wherein a        representative sample of seed of NUN 89007 PPS is deposited        under Accession Number NCIMB 43268;    -   b) selecting a seed, a plant or a plant part or a cell of NUN        89007 PPS, having a mutation; and    -   c) optionally growing and/or multiplying the seed, plant or        plant part or cell of

NUN 89007 PPS, having the mutation.

The disclosure also provides a plant having one, two or threephysiological and/or morphological characteristics which are differentfrom those of NUN 89007 PPS and which otherwise has all thephysiological and morphological characteristics of said variety, whereina representative sample of seed of variety NUN 89007 PPS is depositedunder Accession Number NCIMB 43268. In particular, variants which differfrom NUN 89007 PPS, in none, one, two or three of the characteristicsmentioned in Table 1 and/or 2 and/or 3 are encompassed.

A part of NUN 89007 PPS (or of progeny of said variety or of a planthaving all physiological and/or morphological characteristics but one,two or three which are different from those of said variety) encompassesany cells, tissues, organs obtainable from the seedlings or plants, suchas but not limited to: a pepper fruit or a part thereof, a cutting,hypocotyl, cotyledon, seed coat, pollen and the like. Such parts can bestored and/or processed further. The disclosure further provides forfood or feed products comprising a part of NUN 89007 PPS, or a part ofprogeny of said variety, or a part of a plant having all but one, two orthree physiological and/or morphological characteristics of NUN 89007PPS, comprising one or more of such parts, optionally processed (such ascanned, chopped, cooked, roasted, in a sauce, in a sandwich, pasted,puréed or concentrated, juiced, frozen, dried, pickled, or powdered).

In one aspect, the disclosure provides for a haploid plant and/or adoubled haploid plant of NUN 89007 PPS, or of a plant having all butone, two or three physiological and/or morphological characteristics ofNUN 89007 PPS, or progeny of any of these. Haploid and doubled haploid(DH) plants can, for example, be produced by cell or tissue culture andchromosome doubling agents and regeneration into a whole plant. DHproduction chromosome doubling may be induced using known methods, suchas colchicine treatment or the like). In one aspect, the methodcomprises inducing a cell or tissue culture with a chromosome doublingagent and regenerating the cells or tissues into a whole plant.

In another aspect, the disclosure comprises a method for making doubledhaploid cells from haploid cells of NUN 89007 PPS comprising doublingcells of with a doubling agent, such as a colchicine treatment (see,e.g., Nikolova V, Niemirowicz-Szczytt K (1996) Acta Soc Bot Pol65:311-317).

In yet another aspect, the disclosure provides for haploid plants and/ordoubled haploid plants derived from NUN 89007 PPS that, when combined,make a set of parents of NUN 89007 PPS. The haploid plant and/or thedoubled haploid plant of NUN 89007 PPS can be used in a method forgenerating parental lines of NUN 89007 PPS.

Using methods known in the art such as “reverse synthesis of breedinglines” or “reverse breeding”, it is possible to produce parental linesfor a hybrid plant such as NUN 89007 PPS. Thus, a skilled person cantake any individual heterozygous plant (called a “phenotypicallysuperior plant” in Example 2 of US2015/0245570, hereby incorporated byreference in its entirety; NUN 89007 PPS is such plant) and generate acombination of parental lines (reverse breeding parental lines) that,when crossed, produce NUN 89007 PPS. It is not necessary that thereverse breeding parental lines are identical to the original parentallines. Such breeding methods are based on the segregation of individualalleles in the spores produced by a desired plant and/or in the progenyderived from the self-pollination of that desired plant, and on thesubsequent identification of suitable progeny plants in one generation,or in a limited number of inbred cycles. Such a method is known fromUS2015/0245570 or from Wijnker et al., Nature Protocols Volume: 9,Pages: 761-772 (2014) DOI: doi:10.1038/nprot.2014.049. Thus, thedisclosure provides a method for producing parental lines for a hybridorganism (e.g., NUN 89007 PPS), comprising in one aspect: a) defining aset of genetic markers present in a heterozygous form (H) in a partiallyheterozygous starting organism; b) producing doubled haploid lines fromspores of the starting organism; c) genetically characterizing thedoubled haploid lines thus obtained for the said set of genetic markersto determine whether they are present in a first homozygous form (A) orin a second homozygous form (B); and d) selecting at least one pair ofdoubled haploid lines that have complementary alleles for at least asubset of the genetic markers, wherein each member of the pair issuitable as a parental line for the hybrid organism.

In another aspect, the method for producing parental lines for hybridorganisms, e.g., of NUN 89007 PPS, which when crossed reconstitute thegenome of NUN 89007 PPS comprising:

-   -   a) defining a set genetic markers that are present a        heterozygous form (H) in a partially heterozygous starting        organism;    -   b) producing at least one further generation from the starting        organism by self-pollination (e.g., F2 or F3 generation);    -   c) selecting at least one pair of progeny organisms in which at        least one genetic marker from the set is present in a        complementary homozygous forms (B vs. A, or A vs. B); and    -   d) optionally repeating steps b) and c) until at least one pair        of progeny organisms that have complementary alleles for at        least a subset of the genetic markers has been selected as        parental lines for a hybrid

The disclosure also provides a method for producing parental lines forhybrid NUN 89007 PPS comprising: genetically characterizing a doubledhaploid line from NUN 89007 PPS to determine whether one or more geneticmarkers are present in a first homozygous form or in a second homozygousform in said line, wherein the one or more genetic markers are presentin a heterozygous form in NUN 89007 PPS; and selecting at least one pairof doubled haploid lines that have complementary alleles for the one ormore the genetic markers, wherein each member of the pair is suitable asa parental line for a hybrid organism, optionally this method furthercomprises defining a set of genetic markers present in a heterozygousform in NUN 89007 PPS; and producing doubled haploid lines from NUN89007 PPS. Doubled haploid lines generated as described herein can beused in such a method.

Thus, in one aspect, the disclosure relates to a method of producing acombination of parental lines of a plant of NUN 89007 PPS comprisingmaking doubled haploid cells from haploid cells from said plant or aseed of that plant; and optionally crossing these parental lines toproduce and collect seeds. In another aspect, the disclosure relates toa combination of parental lines produced by this method. In stillanother aspect, the combination of parental lines can be used to producea seed or plant of NUN 89007 PPS, when these parental lines are crossed.In still another aspect, the disclosure relates to a combination ofparental lines from which a seed or plant having all physiologicaland/or morphological characteristics of NUN 89007 PPS (when thecharacteristics are determined at the 5% significance level for plantsgrown under the same conditions).

In another aspect, the disclosure provides a method of introducing asingle locus conversion or single trait conversion or a desired traitinto NUN 89007 PPS comprising:

-   -   a) obtaining a combination of a parental lines of NUN 89007 PPS,        optionally through reverse synthesis of breeding lines;    -   b) introducing a single locus conversion in at least one of the        parents of step a; and    -   c) crossing the converted parent with the other parent of step a        to obtain seed of

NUN 89007 PPS.

A combination of a male and a female parental line of NUN 89007 PPS canbe generated by methods described herein, for example, through reversesynthesis of breeding lines.

In another aspect, the disclosure provides a method of introducing asingle locus conversion or single trait conversion or a desired traitinto NUN 89007 PPS, comprising introducing a single locus conversion inat least one of the parents of NUN 89007 PPS; and crossing the convertedparent with the other parent of NUN 89007 PPS to obtain seed of NUN89007 PPS.

The disclosure also provides a combination of parental lines which, whencrossed, produce a seed or plant having all physiological and/ormorphological characteristics of NUN 89007 PPS but one, two or threewhich are different (when grown under the same environmentalconditions), as well as a seed or plant having all physiological and/ormorphological characteristics of NUN 89007 PPS but one, two or threewhich are different (when the characteristics are determined at the 5%significance level for plants grown under the same conditions).

In another aspect, the step of introducing a single locus conversion inat least one of the parents comprises:

-   -   i. obtaining a cell or tissue culture of cells of the parental        line of NUN 89007 PPS;    -   ii. genetically transforming or mutating said cells;    -   iii. growing the cells into a plant; and    -   iv. optionally selecting plants that contain the single locus        conversion, the single trait conversion or the desired trait.

In another method, the step of introducing a single locus conversion inat least one of the parents comprises genetically transforming ormutating cells the parental line of NUN 89007 PPS; growing the cellsinto a plant; and optionally selecting plants that contain the singlelocus conversion, the single trait conversion or the desired trait.

In another aspect, the step of introducing a single locus conversion inat least one of the parents comprises:

-   -   i. crossing the parental line of NUN 89007 PPS, with a second        pepper plant comprising the single locus conversion, the single        trait conversion or the desired trait;    -   ii. selecting F1 progeny plants that contain the single locus        conversion, the single trait conversion or the desired trait;    -   iii. crossing said selected progeny plants of step ii with the        parental line of step i, to produce a backcross progeny plant;    -   iv. selecting backcross progeny plants comprising the single        locus conversion, the single trait conversion or the desired        trait and otherwise all or all but one, two or three of the        morphological and physiological characteristics the parental        line of step i to produce selected backcross progeny plants; and    -   v. optionally repeating steps iii and iv one or more times in        succession to produce selected second, third or fourth or higher        backcross progeny plants comprising the single locus conversion,        the single trait conversion or the desired trait and otherwise        all or all but one, two or three of the morphological and        physiological characteristics the parental line of step i to        produce selected backcross progeny plants, when grown in the        same environmental conditions.        The disclosure further relates to plants obtained by this        method.

In the above methods, wherein the single locus conversion concerns atrait, the trait may be yield or pest resistance or disease resistance.In one aspect, the trait is disease resistance and the resistance isconferred to Cucumber Mosaic Virus, Curly Top Virus, Pepper Mild MottleVirus, Potato Y Virus, Tobacco Etch Virus, Tobacco Mosaic Virus, TomatoSpotted Wilt Virus, Anthracnose (Gloeosporium piperatum), Bacterial Spot(Xanthomonas vesicatoria), Cercospora Leaf Spot (Cercospora capsici),Nematode (Meloidogyne incognita acrita), Phytophthora Root Rot(Phytophthora capsici), Ripe Rot (Vermicularia capsici), Southern Blight(Sclerotium rolfsii) and/or Verticillium Wilt (Verticillium dahliae).Other resistance genes, against pathogenic viruses, fungi, bacteria,nematodes, insects or other pests may also be introduced. In anotheraspect, the resistance is TSWV resistance.

Also provided is a plant part obtainable from variety NUN 89007 PPS, orfrom progeny of said variety or from a plant having all but one, two orthree physiological and/or morphological characteristics which aredifferent from those of said variety, or from a vegetatively propagatedplant of NUN 89007 PPS (or from its progeny or from a plant having allor all but one, two or three physiological and/or morphologicalcharacteristics which are different from those of said variety): afruit, a harvested fruit, a part of a fruit, a leaf, a part of a leaf,pollen, an ovule, a cell, a petiole, a shoot or a part thereof, a stemor a part thereof, a root or a part thereof, a root tip, a cutting, aseed, a part of a seed, seed-coat or another maternal tissue which ispart of a seed grown on NUN 89007 PPS, or hypocotyl, cotyledon, a scion,a stock, a rootstock, a pistil, an anther, and a flower or a partthereof.

In another aspect, the disclosure provides a method of determining thegenotype of a plant of the disclosure comprising detecting in the genome(e.g., a sample of nucleic acids) of the plant at least a firstpolymorphism or an allele. The skilled person is familiar with manysuitable methods of genotyping, detecting a polymorphism or detecting anallele including SNP (Single Nucleotide Polymorphism) genotyping,restriction fragment length polymorphism identification (RFLP) ofgenomic DNA, random amplified polymorphic detection (RAPD) of genomicDNA, amplified fragment length polymorphism detection (AFLP), polymerasechain reaction (PCR), DNA sequencing, allele specific oligonucleotide(ASO) probes, and hybridization to DNA microarrays or beads.Alternatively, the entire genome could be sequenced. The method may, incertain aspects, comprise detecting a plurality of polymorphisms in thegenome of the plant, for example by obtaining a sample of nucleic acidfrom a plant and detecting in said nucleic acids a plurality ofpolymorphisms. The method may further comprise storing the results ofthe step of detecting the plurality of polymorphisms on a computerreadable medium.

The disclosure also provides for a food or feed product comprising orconsisting of a plant part described herein. Particularly, the plantpart is a pepper fruit or part thereof and/or an extract from a fruit oranother plant part described herein. The food or feed product may befresh or processed, e.g., dried, grinded, powdered, pickled, chopped,cooked, roasted, in a sauce, in a sandwich, pasted, puréed orconcentrated, juiced, pickled, canned, steamed, boiled, fried, blanchedand/or frozen, etc.

Marketable pepper fruits are generally sorted by size and quality afterharvest. Alternatively, the pepper fruits can be sorted by expectedshelf life, pH or Brix.

NUN 89007 PPS may also be grown for use as rootstocks (stocks) orscions. Typically, different types of peppers are grafted to enhancedisease resistance, which is usually conferred by the rootstock, whileretaining the horticultural qualities usually conferred by the scion. Itis not uncommon for grafting to occur between cultivated peppervarieties and related pepper species. Methods of grafting and vegetativepropagation are well-known in the art.

In another aspect, the disclosure relates to a plant comprising arootstock or scion of NUN 89007 PPS.

All documents (e.g., patent publications) are herein incorporated byreference in their entirety, including the following cited references:

-   UPOV, “Guidelines for the Conduct of Tests for Distinctness,    Uniformity and Stability, TG/76/8 (Geneva 2006, revised 2015-03-25);    world wide web at upov.int/ under edocs/tgdocs/en/tg076. pdf-   US Department of Agriculture, Agricultural Marketing Service,    “Objective Plant Description of Variety Pepper (Capsicum spp.)”    world wide web at ams.    usda.gov/services/plant-variety-protection/pvpo-c-forms, under    pepper.-   Acquaah, Principles of Plant Genetics and Breeding, 2007, Blackwell    Publishing, ISBN-13: 978-1-4051-3646-4.-   Ince, A. G., et al., “Genetic Relationship Within and Between    Capsicum Species”, Biochem Genet, 2010, vol. 48, pp. 83-95.-   Kothari, S. L., et al., “Chili Peppers—A review on Tissue Culture    and Transgenesis”, Biotechnology Advances, 2010, vol. 28, pp. 35-48.-   Martin, E., et al., “Identification of Markers Linked to Agronomic    Traits in Globe Artichoke”, Australian Journal of Crop Science, vol.    1, no. 2, pp. 43-46.-   Needleman, S. B., et. al., “A General Method Applicable to the    Search for Similarities in the Amino Acid Sequence of Two Proteins”,    Journal of Molecular Biology, 1970, vol. 48(3), pp. 443-53.-   Nikolova, V., et. al., “Diploidization of Cucumber (Cucumis sativus    L.) Haploids by Colchini Treatment”, Acta Societas Botanicorum    Poloniae, 1996, vol. 65, pp. 311-317.-   Rice, P., et al., “EMBOSS: The European Molecular Biology Open    Software Suite”, Trends in Genetics, 2000, vol. 16, Issue 6. pp.    276-277.-   Sang-Gu, K., et al., “Callus Growth and Plant Regeneration in    Diverse Cultivars of Cucumber”, Plant Cell, Tissue and Organ    Culture, 1988, vol. 12, pp. 67-74.-   Tiwari, A., et al., “Parthenocarpic Potential in Capsicum annum L.    is Enhanced by Carpelloid Structures and Controlled by Single    Recessive Gene”, BMC Plant Biology, 2011, vol. 11, pp. 2-14, DOI:    10.1186/1471-2229-11-143-   Vos, P., et al., AFLP: A New Technique for DNA Fingerprinting 1995,    Nucleic Acids Research, 1995, vol. 23, No. 21, pp. 4407-4414.-   Wijnker, E., et al., Hybrid Recreation by Reverse breeding in    Arabidopsis thaliana, Nature Protocols, 2014, vol. 9, pp. 761-772.    DOI: doi: 10.1038/nprot.2014.049-   U.S. Pat. No. 8,492,619-   US2006/0037100-   US2015/0245570-   US2015/0126380-   https://www.ams.usda.gov/resources/st470-pepper-   https://anrcatalog.ucanr.edu/pdf/7217.pdf-   http://www.upov.int/edocs/tgdocs/en/tg076_08.pdf-   https://www.wifss.ucdavis.edu/wp-content/uploads/2016/10/Peppers_PDF.pdf

EXAMPLES Development of NUN 89007 PPS

The hybrid NUN 89007 PPS was developed from a male and femaleproprietary inbred line of Nunhems. The female and male parents werecrossed to produce hybrid (F1) seeds of NUN 89007 PPS. The seeds of NUN89007 PPS can be grown to produce hybrid plants and parts thereof (e.g.,pepper fruit). The hybrid NUN 89007 PPS can be propagated by seeds orvegetative.

The hybrid variety is uniform and genetically stable. This has beenestablished through evaluation of horticultural characteristics. Severalhybrid seed production events resulted in no observable deviation ingenetic stability. Coupled with the confirmation of genetic stability ofthe female and male parents the Applicant has concluded that NUN 89007PPS is uniform and stable.

Deposit Information

A total of 2500 seeds of the hybrid variety NUN 89007 PPS has beendeposited according to the Budapest Treaty by Nunhems B.V. on November13, 2018, at the NCIMB Ltd., Ferguson Building, Craibstone Estate,Bucksburn, Aberdeen AB21 9YA, United Kingdom (NCIMB). The deposit isassigned NCIMB Number 43268. A deposit of NUN 89007 PPS and of the maleand female parent line is also maintained at Nunhems B.V.

Access to the deposits will be available during the pendency of thisapplication to persons determined by the Director of the U.S. PatentOffice to be entitled thereto upon request. Subject to 37 C.F.R. §1.808(b), all restrictions imposed by the depositor on the availabilityto the public of the deposited material will be irrevocably removed uponthe granting of the patent. The deposit will be maintained for a periodof 30 years, or 5 years after the most recent request, or for theenforceable life of the patent whichever is longer and will be replacedif it ever becomes nonviable during that period. Applicant does notwaive any rights granted under this patent on this application or underthe Plant Variety Protection Act (7 U.S.C. § 2321 et seq.).

The most similar variety to NUN 89007 PPS is referred to as ReferenceVariety, a variety from ______ with the commercial name ______. In Table1 and/or 2, a comparison between NUN 89007 PPS and the Reference Varietywill be shown based on a trial in the USA during the trial season 2018.Trial location ______, transplanting date: ______, harvesting date:______.

A trial of 30 plants of each variety, from which at least 15 plants orplant parts will be randomly selected, and used to measurecharacteristics. For numerical characteristics averages will becalculated. For non-numerical characteristics the type/degree will bedetermined. In Table 1 and/or 2, the USDA and Non-USDA descriptors ofNUN 89007 PPS and the Reference Variety are listed, which will bemeasured in the trial to be performed.

In one aspect, the disclosure provides a plant having the physiologicaland morphological characteristics of NUN 89007 PPS, as will be presentedin Table 1 and/or 2.

TABLE 1 Objective description of NUN 89007 PPS and the Reference Variety(USDA Descriptors); significant differences are highlighted in bold,where quantitative values are mentioned these are statisticallydifferent between NUN 89007 PPS and the Reference Variety using an ANOVATukey test. USDA Descriptors NUN 89007 PPS Reference Variety Species:

1 = C. annuum; 2 = C. frutescens; 3 = C. baccatum; 4 = C. chinense; 5 =Other (specify) Maturity (in Region of Best Adaptability) Days fromtransplanting until mature green stage Days from transplanting untilmature red or yellow stage Days from direct seeding until mature greenstage Days from direct seeding until mature red or yellow stage PlantPlant Habit: 1 = Compact; 2 = Semi-spreading; 3 = Spreading; 4 = OtherPlant Attitude: 1 = Erect; 2 = Semi-erect; 3 = Prostrate 4 = Other PlantHeight (cm) Plant Width (cm) Length of Stem from Cotyledons to FirstFlower (cm) Length of Third Internode (from soil surface) (mm) BasalBranches: 1 = None; 2 = Few (2-3); 3 = Many (more than 3) BranchFlexibility: 1 = Willowy (Cayenne Long Red); 2 = Rigid (Yolo Wonder L)Stem Strength (Breakage Resistance): 1 = Weak; 2 = Intermediate; 3 =Strong Leaves Leaf Width (mm) Leaf Length (mm) Petiole Length (mm)Mature Leaf Shape: 1 = Lanceolate; 2 = Elliptic Leaf Color: 1 = LightGreen; 2 = Medium Green; 3 = Dark Green; 4 = Purple; 5 = Other (specify)(RHS color chart value) Leaf and Stem Pubescence: 1 = Absent (YoloWonder L); 2 = Light; 3 = Moderate (Serrano); 4 = Heavy (Chili Piquin)Margin Undulation: 1 = Absent; 2 = Very Weak; 3 = Weak 4 = Medium; 5 =Strong; 6 = Very Strong Blistering: 1 = Absent; 2 = Very Weak; 3 = Weak4 = Medium; 5 = Strong; 6 = Very Strong Flowers Number of Flowers perLeaf Axil Number of Calyx Lobes Number of Petals Flower Diameter (mm)Corolla Color: 1 = White; 2 = Purple; 3 = Other (Specify) Corolla ThroatMarkings: 1 = Yellow (Tan); 2 = Purple; 3 = Other (Specify) AntherColor: 1 = Yellow; 2 = Purple; 3 = Other (Specify) Style Length: 1 =Less Than Stamen; 2 = Same as Stamen; 3 = Exceeds StamenSelf-Incompatibility: 1 = Absent; 2 = Present Fruit Group: 1 = Bell(Yolo Wonder L); 2 = Pimiento (Pimiento Perfection); 3 = Ancho (MexicanChili); 4 = Anaheim Chili (Sandia); 5 = Cayenne (Cayenne Long Red); 6 =Cuban (Cubanelle); 7 = Jalapeno (Jalapeno); 8 = Small Hot (Serrano); 9 =Cherry (Sweet Cherry); 10 = Short Wax (Floral Gem); 11 = Long Wax (SweetBanana); 12 = Tabasco (Tabasco); 13 = Habanero (Scotch Bonnet); 14 =Other Immature Fruit Color: 1 = Light Green (Cubanelle); 2 = MediumGreen (Long Thin Cayenne); 3 = Dark Green (Yolo Wonder L); 4 = Very DarkGreen (Ancho Chili); 5 = Yellow (Yellow Belle); 6 = Purple (Violetta); 7= Ivory (Twiggy); 8 = Other (RHS color chart value) Mature Fruit Color:1 = Red (Yolo Wonder L); 2 = Orange; 3 = Orange-Yell ow (GoldenCalwonder); 4 = Brown (Mulatto); 5 = Ivory; 6 = Green (Permagreen); 7 =Salmon; 8 = Lemon Yellow; 9 = Other (RHS color chart value) Pungency: 1= Sweet (Yolo Wonder L); 2 = Hot (Jalapeno) mg Capsaicin per gram dryfruit Scoville Units (dry fruit) Flavor: 1 = Mild Pepper Flavor; 2 =Moderate Pepper Flavor; 3 = Strong Pepper Flavor; 4 = Other FruitGlossiness: 1 = Dull; 2 = Moderate; 3 = Shiny Surface Smoothness: 1 =Smooth (Yolo Wonder L); 2 = Rough (Long Thin Cayenne) Fruit Position: 1= Upright (Santaka); 2 = Horizontal; 3 = Pendent (Jalapeno) Calyx Shape:1 = Cup-shaped (Enveloping Fruit Base); 2 = Saucer-shaped (Flat,Non-Enveloping) Calyx Diameter (mm) Fruit Length (mm) Fruit Diameter atCalyx Attachment (mm) Fruit Diameter at Mid-point (mm) Flesh Thicknessat Mid-point (mm) Average Number of Fruits per Plant % Large fruits(Weight range: _ to _) % Medium fruits (Weight range: _ to _) % smallfruits (Weight range: _ to _) Average Fruit Weight (gm) Fruit BaseShape: 1 = Cupped (Yolo Wond L); 2 = Rounded (Jalapeno) Fruit ApexShape: 1 = Pointed (Long Thin Cayenne); 2 = Blunt (Yolo Wonder L) FruitShape: 1 = Bell (Yolo Wonder L); 2 = Conical (Pimiento); 3 = Elongate(Long Thin Cayenne); 4 = Oblong (Jalapeno) 5 = Oblate (Surmybrook); 6 =Globe (Red Cherry); 7 = Other Fruit Shape (Longitudinal Section, seeattached pictures): 1 = Flattened; 2 = Round; 3 = He art- shaped; 4 =Square; 5 = Rectangular; 6 = Trapezoid; 7 = Narrow Triangular 8 =Triangular; 9 = Horn-shaped Fruit Shape (Cross Section, at Level ofPlacenta): 1 = Elliptic; 2 = Triangular; 3 = Quadrangular; 4 = CircularFruit Set: 1 = Scattered; 2 = Concentrated Interloculary Grooves: 1 =Absent; 2 = Very Shallow; 3 = Shallow; 4 = Medium; 5 = Deep; 6 = VeryDeep % Fruits with one locule % Fruits with two locules % Fruits withthree locules % Fruits with four locules % Fruits with five or morelocules Average Number of Locules Pedicel Length (mm) Pedicel Thickness(mm) Pedicel Shape: 1 = Straight; 2 = Curved Pedicel Cavity: 1 = Absent;2 = Present Seed Seed Cavity Length (mm) Seed Cavity Diameter (mm)Placenta Length (mm) Number of Seeds per Fruit Gm per 1000 seeds SeedColor: 1 = Yellow; 2 = Purple Anthocyanin (1 = Absent; 2 = Weak; 3 =Moderate; 4 = Strong) Seedling hypocotyl Stem Node Leaf Pedicel CalyxFruit

indicates data missing or illegible when filed

TABLE 2 Objective description of NUN 89007 PPS and the Reference Variety(Non-USDA descriptors); significant differences are highlighted in bold,where quantitative values are mentioned these are statisticallysignificantly different between NUN 89007 PPS and the Reference Varietyusing an ANOVA Tukey test Non-USDA Descriptors NUN 89007 PPS ReferenceVariety

indicates data missing or illegible when filed

TABLE 3 UPOV Characteristics UPOV Characteristics NUN 89007 PPSSeedling: anthocyanin coloration of hypocotyl 9 (1 absent/9 present)Plant: habit (1 upright/2 semi-upright/3 prostrate) 1 Plant: shortenedinternode (in upper part) (1 absent/9 1 present) Plant: number offlowers per node (1 one or two/2 three 1 or more) Varieties withshortened internodes only: 1 Plant: number of internodes between thefirst flower and shortened internodes (1 none/2 one to three/3 more thanthree) Plant: anthocyanin coloration of nodes (1 absent/9 9 present)Plant: vigour (1 very weak/3 weak/5 medium/7 strong/ 7 9 very strong)Stem: intensity of anthocyanin coloration of nodes (1 5 very weak/3weak/5 medium/7 strong/9 very strong) Stem: hairiness of nodes (1 absentor very weak/3 weak/ 1 5 medium/7 strong/9 very strong) Leaf: color (1dark green/2 green/3 yellow green) 2 Leaf: intensity of green color (1very light/3 light/5 7 medium/7 dark/9 very dark) Leaf: shape (1lanceolate/2 ovate/3 broad elliptic) 2 Leaf: undulation of margin (1absent or very weak/3 5 weak/5 medium/7 strong/9 very strong) Leaf:pubescence (1 absent/9 present) 1 Leaf: blistering (1 very weak/3 weak/5medium/7 3 strong/9 very strong) Leaf: profile in cross section (1strongly concave/2 2 moderately concave/3 flat/4 moderately convex/5strongly convex) Leaf: glossiness (1 very weak/3 weak/5 medium/7 3strong/9 very strong) Peduncle: attitude (1 erect/2 semi-drooping/3drooping) 3 Flower: attitude of peduncle (1 erect/2 non-erect) 2 Flower:color before maturity (1 greenish white/2 3 yellow/3 green/4 purple)Flower: Anther color (3 yellow/5 pale blue/7 purple/9 5 others) Flower:days to 50% flowering (from the date of sowing) 5 (3 early/5 medium/7late) Flower: anthocyanin coloration in anther (1 absent/9 9 present)Fruit: color (before maturity) (1 greenish white/2 3 yellow/3 green/4purple) Fruit: intensity of color (before maturity) (1 very light/3 7light/5 medium/7 dark/9 very dark) Bearing habit: Number of fruits pernode (3 Solitary 3 (only 1)/5 two to three/7 more than three) Fruit:anthocyanin coloration (1 absent/9 present) 9 Fruit: attitude (1 erect/2horizontal/3 drooping) 3 Fruit: shape in longitudinal section (1oblate/2 circular/ 7 3 cordate/4 square/5 rectangular/6 trapezoidal/7moderately triangular/8 narrowly triangular/9 hornshaped) Fruit:curvature (1 absent/9 present) 1 Fruit: shape in cross section (at levelof placenta) (1 3 elliptic/2 angular/3 circular) Fruit: sinuation ofpericarp at basal part (1 absent or very 1 weak/3 weak/5 medium/7strong/9 very strong) Fruit: sinuation of pericarp excluding basal part(1 absent 1 or very weak/3 weak/5 medium/7 strong/9 very strong) Fruit:texture of surface (1 smooth or very slightly 1 wrinkled/2 slightlywrinkled/3 strongly wrinkled) Fruit: color (Lt maturity) (1 yellow/2orange/3 red/4 2 brown/5 green) Fruit: intensity of color (at maturity)(1 very light 3 light/ 5 5 medium/7 dark/9 very dark) Fruit: colortransition (3 one stage/5 two stages7 more 3 than two stages) Fruit:glossiness (1 very weak/3 weak/5 medium/7 7 strong/9 very strong) Fruit:shape of base (3 acute/5 round/7 sunken) 5 Fruit: stalk cavity (1absent/9 present) 1 Fruit: shape of apex (1 very acute/2 moderatelyacute/3 2 rounded/4 moderately depressed/5 very depressed) Fruit: depthof interloculary grooves (1 absent or very 1 shallow/3 shallow/5medium/7 deep) Fruit: number of locules (1 predominantly two/2 equally 2two and three/3 predominantly three/4 equally three and four/5predominantly four and more) Calyx: aspect (1 non enveloping/2enveloping) 1 Fruit: capsaicin in placenta (1 absent/9 present) 1 Fruit:firmness (1 very weak/3 weak/5 medium/7 5 strong/9 very strong) Time ofbeginning of flowering (first flower on second 5 flowering node) (3early/5 medium/7 late) Time of maturity (1 very early/3 early/5 medium/7late/ 5 9 very late)

Tables 1, 2 and 3 contain typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the disclosure. N.A.=not applicable; n.r.=notrecorded.

1. A plant, a plant part or a seed of pepper variety NUN 89007 PPS,wherein a representative sample of seed of said pepper variety NUN 89007PPS is deposited under Accession Number NCIMB
 43268. 2. The plant partof claim 1, wherein said plant part is a fruit, a leaf, pollen, anovule, a cell, a scion, a root, a rootstock, a cutting, a flower or apart of any of these.
 3. A seed that produces the plant of claim
 1. 4. Aseed grown on the plant of claim
 1. 5. A pepper plant, or a part thereofwhich does not significantly differ from the plant of claim 1 in all ofthe following distinguishing characteristics in Table 1 and/or 2: 1)______; 2) ______; 3) ______; 4) ______; 5) ______; 6) ______; 7)______; 8) ______; 9) ______; and 10) ______, when the characteristicsare determined at the 5% significance level when grown under the sameenvironmental conditions.
 6. A pepper plant, or a part thereof whichdoes not differ from the plant of claim 1 in all of the distinguishingcharacteristics of Table 1 and/or 2, when the characteristics aredetermined at the 5% significance level when grown under the sameenvironmental conditions.
 7. A tissue or cell culture comprising cellsof the plant of claim
 1. 8. The tissue or cell culture according toclaim 7, comprising cells or protoplasts derived from a plant partsuitable for vegetative reproduction.
 9. The tissue or cell cultureaccording to claim 7, wherein the plant part is an embryo, a meristem, acotyledon, a hypocotyl, pollen, a leaf, an anther, a root, a root tip, apistil, a petiole, a flower, a fruit, a seed, a stem, or a stalk.
 10. Apepper plant regenerated from the tissue or cell culture of claim 7,wherein the regenerated plant has all or all but one, two, or three ofthe physiological and morphological characteristics of NUN 89007 PPS,when the characteristics are determined at the 5% significance level forplants grown under the same environmental conditions, and wherein arepresentative sample of seed of pepper variety NUN 89007 PPS isdeposited under Accession Number NCIMB
 43268. 11. A method of producingthe plant of claim 1, or a part thereof, comprising vegetativepropagation of NUN 89007 PPS, wherein a representative sample of seed ofsaid pepper variety NUN 89007 PPS is deposited under Accession NumberNCIMB
 43268. 12. The method of claim 11, wherein said vegetativepropagation comprises regenerating a whole plant from said part of NUN89007 PPS, wherein a representative sample of seed of said peppervariety NUN 89007 PPS is deposited under Accession Number NCIMB 43268.13. The method of claim 11, wherein said part is a cutting, a cellculture or a tissue culture.
 14. A vegetative propagated plant of NUN89007 PPS, or a part thereof, wherein the vegetative propagated planthas all or all but one, two, or three of the physiological andmorphological characteristics of the plant of NUN 89007 PPS, when thecharacteristics are determined at the 5% significance level for plantsgrown under the same conditions, and wherein a representative sample ofseed of NUN 89007 PPS is deposited under Accession Number NCIMB 43268.15. A method of producing a pepper plant, comprising crossing the plantof claim 1 with a second pepper plant at least once, allowing theprogeny to form seed and optionally selecting progeny from saidcrossing.
 16. A progeny plant of the plant of claim 1, obtained byselfing NUN 89007 PPS or cross-pollinating NUN 89007 PPS with anotherpepper plant, wherein said progeny plant has all or all but one, two orthree of the physiological and morphological characteristics of theplant of NUN 89007 PPS, when the characteristics are determined at the5% significance level for plants grown under the same environmentalconditions, and a representative sample of seed of said pepper varietyNUN 89007 PPS is deposited under Accession Number NCIMB
 43268. 17. Apepper plant having one, two or three physiological and/or morphologicalcharacteristics which are different from those of the plant of NUN 89007PPS, and which otherwise has all the physiological and morphologicalcharacteristics of the plant of NUN 89007 PPS as listed in Table 1and/or 2, when the characteristics are determined at the 5% significancelevel for plants grown under the same environmental conditions.
 18. Theplant of claim 17, where the different characteristic is affected by amutation or by transformation.
 19. The plant of claim 1, furthercomprising a single locus conversion, wherein said plant has all or allbut one, two or three of the morphological and physiologicalcharacteristics of the plant of NUN 89007 PPS, when said characteristicsare determined at the 5% significance level for plants grown under thesame environmental conditions, and wherein a representative sample ofseed of said pepper variety NUN 89007 PPS is deposited under AccessionNumber NCIMB
 43268. 20. A method of making doubled haploid cells of NUN89007 PPS, comprising making double haploid cells from the plant or seedof NUN 89007 PPS, wherein a representative sample of seed of said peppervariety NUN 89007 PPS is deposited under Accession Number NCIMB 43268.21. A method of producing a combination of parental lines of the plantof claim 1, comprising: a. genetically characterizing a doubled haploidline from NUN 89007 PPS to determine whether one or more genetic markersare present in a first homozygous form or in a second homozygous form insaid line, wherein the one or more genetic markers are present in aheterozygous form in NUN 89007 PPS; and b. selecting at least one pairof doubled haploid lines that have complementary alleles for the one ormore the genetic markers, wherein each member of the pair is suitable asa parental line for NUN 89007 PPS.
 22. The method of claim 21, furthercomprising: i. defining a set of genetic markers present in aheterozygous form in NUN 89007 PPS; and ii. producing doubled haploidlines from NUN 89007 PPS.
 23. A plant comprising the scion or rootstockof claim
 2. 24. A container comprising a plant, a plant part or a seedof claim
 1. 25. A food or feed product or a processed product comprisingthe plant part of claim
 2. 26. A method of producing a pepper fruit,comprising growing NUN 89007 PPS until it sets at least one fruit, andcollecting the fruit.
 27. A method for inducing a mutation in the plantof claim 1, comprising: a. exposing a seed, a plant or a plant part ofNUN 89007 PPS to a mutagenic compound or to radiation, wherein arepresentative sample of seed of said pepper variety NUN 89007 PPS isdeposited under Accession Number NCIMB; and b. selecting a seed, a plantor a plant part or a cell of NUN 89007 PPS, having a mutation.
 28. Amethod for collecting pollen of NUN 89007 PPS comprising growing a plantof claim 1 until at least one flower contains pollen and collecting thepollen.
 29. The plant of pepper variety NUN 89007 PPS of claim 19,wherein the single locus conversion confers male sterility, herbicidetolerance, pest resistance, environmental stress resistance, modifiedcarbohydrate metabolism, modified protein metabolism or ripening.
 30. Apepper plant comprising at least a first set of the chromosomes of NUN89007 PPS, wherein a representative sample of seed of said peppervariety NUN 89007 PPS is deposited under Accession Number NCIMB 43268,and wherein said pepper plant comprising said first set of thechromosomes has all or all but one, two, or three of the physiologicaland morphological characteristics of NUN 89007 PPS, when thecharacteristics are determined at the 5% significance level for plantsgrown under the same environmental conditions.
 31. A method of producinga pepper plant having a desired trait, wherein the method comprisesmutating a plant of variety NUN 89007 PPS and selecting a plant with adesired trait, wherein the mutated plant retains all or all but one,two, or three of the physiological and morphological characteristics ofNUN 89007 PPS, when the characteristics are determined at the 5%significance level for plants grown under the same environmentalconditions, and wherein a representative sample of seed of said peppervariety NUN 89007 PPS is deposited under Accession Number NCIMB 43268.32. The method of claim 31, wherein the desired trait is male sterility,herbicide tolerance, pest resistance, environmental stress resistance,modified carbohydrate metabolism, modified protein metabolism orripening.